Efficacy of fluorescent microscopy vs modified Fite-Faraco stain in skin biopsy specimens of leprosy cases – A comparative study
International Journal of Dermatology — Kalagarla S, Alluri R, Saka S, et al. | January 31, 2022
Studies from few centers have indicated that in the diagnosis of leprosy, detection of more acid-fast bacilli (AFB) is made using fluorescent microscopy vs modified Fite-Faraco staining; however, this is sparsely documented. In this work, the sensitivity of fluorescent microscopy and modified Fite-Faraco stain in the detection of Mycobacterium leprae in tissue sections has been studied.
Forty patients diagnosed with leprosy using histopathology were recruited.
Staining of tissue sections was done by fluorescent stain and modified Fite-Faraco stain and calculation of bacillary index for each case was performed.
Findings suggest greater sensitivity of fluorescent staining than modified Fite-Faraco staining in the detection of AFB in tissue sections.
The fluorescent staining led to detection of more bacilli per field compared with the modified Fite-Faraco method.