Expression profiles of circular RNAs in human papillary thyroid carcinoma based on RNA deep sequencing

OncoTargets and TherapyLv C, Sun W, Huang J, et al. | June 21, 2021

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Using RNA deep sequencing, researchers investigated the expression patterns of circRNAs in papillary thyroid carcinoma (PTC) with the overarching goal of improving early diagnosis rates for individuals with PTC. A total of 16,569 circRNAs were identified in deep sequencing of four PTC pairs and neighboring nontumor tissues; of these circRNAs, 301 were upregulated and 419 were downregulated. The RT-qPCR data indicated downregulation of the expression of chr5: 38481299– 38530666-, chr2: 159932176– 159945082-, chr10: 179994– 249088+, chr3: 121378716– 121381532+, and chr1: 237423092– 237445522+; while there was upregulation of the expression of chr4: 25665378– 25667298+, chr5: 161330883– 161336769-, chr1: 12578718– 12579412-, chr7: 116695750– 116700284+, and chr7: 116699071– 116700284+. As per the stability test findings, circRNAs were more tolerant to temperature, RNase R, and time. ROC curves illustrated the following as effective diagnostic indicators: chr4: 25665378– 25667298+, chr1: 12578718– 12579412-, chr7: 116699071– 116700284+, chr7: 116695750– 116700284+, chr5: 161330883– 161336769-, and chr10: 179994– 249088+. Hence, this study suggested the value of several circRNAs as promising PTC diagnostic biomarkers.

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