Detection of HPV infection in urothelial carcinoma using RNAscope: Clinicopathological characterization

Cancer MedicineMusangile FY, Matsuzaki I, Okodo M, et al. | June 25, 2021

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Researchers investigated the clinical and pathological features of human papillomavirus (HPV)-related urothelial carcinoma (UC). Assessment of tissue samples of 228 cases of UC obtained from the bladder, upper and lower urinary tract, and metastatic sites was done to build a tissue microarray. The presence of HPV was analyzed in the samples using a highly sensitive and specific mRNA in situ hybridization (RISH) technique (RNAscope) with a probe that can detect 18 varieties of high-risk HPV. The RISH method was noted to have the highest accuracy and identified HPV infection in 12 (5.2%) of the cases. Overall data indicate a low incidence of HPV infection in UC, however, it is significantly linked with squamous differentiation and poor prognosis.

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